6
   

Does "to" here mean "to join"?

 
 
Reply Wed 11 Jun, 2014 01:51 am

Context:

A synthetic sex ratio distortion system for the control of the human
malaria mosquito

It has been theorized that inducing extreme reproductive sex ratios could be a method to suppress or eliminate pest populations. Limited knowledge about the genetic makeup and mode of action of naturally occurring sex distorters and the prevalence of co-evolving suppressors has hampered their use for control. Here we generate a synthetic sex distortion system by exploiting the specificity of the homing endonuclease I-PpoI, which is able to selectively cleave ribosomal gene sequences of the malaria vector Anopheles gambiae that are located exclusively on the mosquito’s X chromosome. We combine structure-based protein engineering and molecular genetics to restrict the activity of the potentially toxic endonuclease to spermatogenesis. Shredding of the paternal X chromosome prevents it from being transmitted to the next generation, resulting in fully fertile mosquito strains that produce >95% male offspring. We demonstrate that distorter male mosquitoes can efficiently suppress caged wild-type mosquito populations, providing the foundation for a new class of genetic vector control strategies.

MOre:
http://www.nature.com/ncomms/2014/140610/ncomms4977/full/ncomms4977.html
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Type: Question • Score: 6 • Views: 760 • Replies: 6
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View best answer, chosen by oristarA
Joe Nation
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  2  
Reply Wed 11 Jun, 2014 06:38 am
No. 'to' does not mean 'join' in this sentence.

Quote:
We combine structure-based protein engineering and molecular genetics to restrict the activity of the potentially toxic endonuclease to spermatogenesis.


Apparently the endonuclease can create ill effects on several parts of the system, what they have achieved is a method which limits the endonuclease's activity to ONLY spermatogensis. Meaning it will only affect reproduction or the ability to reproduce and nothing else.

Clear?
Joe(it's fascinating work)Nation
oristarA
 
  1  
Reply Wed 11 Jun, 2014 09:08 am
@Joe Nation,
Yes, crystal clear.
Does "Shredding of the paternal X chromosome" mean "removing of the paternal X chromosome"?
oristarA
 
  1  
Reply Wed 11 Jun, 2014 09:57 am
@Joe Nation,
In addition, does "suppressors" (in "Limited knowledge about the genetic makeup and mode of action of naturally occurring sex distorters and the prevalence of co-evolving suppressors " refer to "suppressors of the distorters' action"?
0 Replies
 
oristarA
 
  1  
Reply Wed 11 Jun, 2014 09:57 am
@Joe Nation,
In addition, does "suppressors" (in "Limited knowledge about the genetic makeup and mode of action of naturally occurring sex distorters and the prevalence of co-evolving suppressors " refer to "suppressors of the distorters' action"?
InfraBlue
 
  2  
Reply Wed 11 Jun, 2014 10:26 am
@oristarA,
oristarA wrote:

Yes, crystal clear.
Does "Shredding of the paternal X chromosome" mean "removing of the paternal X chromosome"?

The way I understand it, it refers to the selective cleavage of ribosomal gene sequences of the malaria vector Anopheles gambiae.
0 Replies
 
InfraBlue
 
  2  
Reply Wed 11 Jun, 2014 10:36 am
@oristarA,
oristarA wrote:

In addition, does "suppressors" (in "Limited knowledge about the genetic makeup and mode of action of naturally occurring sex distorters and the prevalence of co-evolving suppressors " refer to "suppressors of the distorters' action"?

At first I read it as two separate phenomenon, but then the "co-evolving" part makes me think that your reading is correct.
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