What does "Cre " stand for?

Forums: English, Esl, Grammar, Efl, Not Grammar
Email this Topic • Print this Page

Context:

A multi-faceted toolkit for all scientists to use

Called "Cre driver lines," Dr. Huang's approach makes use of a well-established and widely used technique called Cre-Lox recombination to create the equivalent of genetic handles in specific types of cells within the cerebral cortex. Different strains of mice have been developed, each to express a particular gene or genes that enable microscopists to home in on particular subtypes of GABA neurons. The key, Huang explains "is that the 'driver' in each case is a gene that we know something about. We know its expression correlates with a subset of GABA neurons. We use that gene as a kind of entry point to express various kinds of markers."

More:

http://www.sciencedaily.com/releases/2011/09/110921132342.htm

Topic Stats
Top Replies
Link to this Topic

Type: Question • Score: 1 • Views: 4,112 • Replies: 8

No top replies

@oristarA,

Do the "lines" in the "Cre driver lines" refer to "the lines of mice"?

0 Replies

@oristarA,

Quote:

Cre recombinase, often abbreviated to Cre, is a Type I topoisomerase from P1 bacteriophage that catalyzes site-specific recombination of DNA between loxP sites. The enzyme does not require any energy cofactors and Cre-mediated recombination quickly reaches equilibrium between substrate and reaction products. The loxP recognition element is a 34 base pair (bp) sequence composed of two 13 bp inverted repeats flanking an 8 bp spacer region which confers directionality. Recombination products are dependent on the location and relative orientation of the loxP sites. Two DNA species containing single loxP sites will be fused whilst DNA between loxP sites in the same orientation will be excised in circular form and DNA between opposing loxP sites will be inverted with respect to the rest of the DNA

LINK

1 Reply

@Ticomaya,

Ticomaya wrote:

Quote:
Cre recombinase, often abbreviated to Cre, is a Type I topoisomerase from P1 bacteriophage that catalyzes site-specific recombination of DNA between loxP sites. The enzyme does not require any energy cofactors and Cre-mediated recombination quickly reaches equilibrium between substrate and reaction products. The loxP recognition element is a 34 base pair (bp) sequence composed of two 13 bp inverted repeats flanking an 8 bp spacer region which confers directionality. Recombination products are dependent on the location and relative orientation of the loxP sites. Two DNA species containing single loxP sites will be fused whilst DNA between loxP sites in the same orientation will be excised in circular form and DNA between opposing loxP sites will be inverted with respect to the rest of the DNA

LINK

Thank you.

Do the "lines" in the "Cre driver lines" refer to "the lines of mice"?

2 Replies

@oristarA,

Plus, in the context (in the first post here), is "Dr. Huang's approach" "Called "Cre driver lines"?

0 Replies

@oristarA,

oristarA wrote:

Do the "lines" in the "Cre driver lines" refer to "the lines of mice"?

I believe so.

1 Reply

@Ticomaya,

Ticomaya wrote:

oristarA wrote:
Do the "lines" in the "Cre driver lines" refer to "the lines of mice"?

I believe so.

　　Thank you.

Plus, in the context (in the first post here), is "Dr. Huang's approach" "Called "Cre driver lines"?

2 Replies

@oristarA,

From Wiki..

Quote:

The Cre (causes recombination) protein consists of 4 subunits and two domains: The larger carboxyl (C-terminal) domain, and smaller amino (N-terminal) domain. The total protein has 343 amino acids. The C domain is similar in structure to the domain in the Integrase family of enzymes isolated from lambda phage. This is also the catalytic site of the enzyme.

.....and the best of luck with that ! Smile

0 Replies

@oristarA,

oristarA wrote:

Plus, in the context (in the first post here), is "Dr. Huang's approach" "Called "Cre driver lines"?

Yes.

0 Replies

What does "Cre " stand for?

Related Topics

Quick Links

My Account

able2know