Reply
Mon 18 Jul, 2011 02:28 am
Context:
We present genome engineering technologies that are capable of fundamentally reengineering genomes from the nucleotide to the megabase scale. We used multiplex automated genome engineering (MAGE) to site-specifically replace all 314 TAG stop codons with synonymous TAA codons in parallel across 32 Escherichia coli strains. This approach allowed us to measure individual recombination frequencies, confirm viability for each modification, and identify associated phenotypes. We developed hierarchical conjugative assembly genome engineering (CAGE) to merge these sets of codon modifications into genomes with 80 precise changes, which demonstrate that these synonymous codon substitutions can be combined into higher-order strains without synthetic lethal effects. Our methods treat the chromosome as both an editable and an evolvable template, permitting the exploration of vast genetic landscapes.
@oristarA,
discrete changes, I would suggest.
@McTag,
exact changes? Unfortunately precise is the best word to use in this context, all other synonyms really don't match up. Look up the term 'precision engineering,' that might help you get your head around it.
@izzythepush,
I don't agree. You wouldn't advocate an imprecise change.
@oristarA,
oristarA wrote:
Context:
We developed hierarchical conjugative assembly genome engineering (CAGE) to merge these sets of codon modifications into genomes with 80 precise changes, which demonstrate that these synonymous codon substitutions can be combined into higher-order strains without synthetic lethal effects.
I would suggest the word '
constituent' would work in this context as it is closer to the meaning of 'integral' than the other suggestions or even '
component'.
@igm,
igm wrote:
oristarA wrote:
Context:
We developed hierarchical conjugative assembly genome engineering (CAGE) to merge these sets of codon modifications into genomes with 80 precise changes, which demonstrate that these synonymous codon substitutions can be combined into higher-order strains without synthetic lethal effects.
I would suggest the word '
constituent' would work in this context as it is closer to the meaning of 'integral' than the other suggestions or even '
component'.
Thanks.
In "these sets of codon modifications into genomes with 80 precise changes," do you agree:
1) The "modifications" refers to " 80 precise changes"?
2) "These sets": each set includes 80 precise changes?
@oristarA,
oristarA wrote:
igm wrote:
oristarA wrote:
Context:
We developed hierarchical conjugative assembly genome engineering (CAGE) to merge these sets of codon modifications into genomes with 80 precise changes, which demonstrate that these synonymous codon substitutions can be combined into higher-order strains without synthetic lethal effects.
I would suggest the word '
constituent' would work in this context as it is closer to the meaning of 'integral' than the other suggestions or even '
component'.
Thanks.
In "these sets of codon modifications into genomes with 80 precise changes," do you agree:
1) The "modifications" refers to " 80 precise changes"?
2) "These sets": each set includes 80 precise changes?
I would say yes, I do agree.
Stumble It!
Tweet This
Bookmark on Delicious
Share on Facebook
Share on MySpace