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Tue 24 Feb, 2015 02:02 am
You want to determine the function of a member of an enzyme family capable of phosphorylating other proteins. You do the following experiments:
1) overexpression in fibroblasts – massive substrate phosphorylation – dramatic phenotype
2) knockdown in fibroblasts – substrate phosphorylation unaffected – dramatic, but different phenotype
3) chemical inhibition – substrate phosphorylation unaffected - no phenotype.
These results don’t match. Any idea why?
Thank's for any help!!!!!!!!!