fayorks
 
Reply Fri 2 May, 2014 07:10 am
In the preparation of blood agar,why do we pour water into the agar and not agar into the water?
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Type: Question • Score: 4 • Views: 1,614 • Replies: 2
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farmerman
 
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Reply Fri 2 May, 2014 07:57 am
@fayorks,
I did some blood protein work years ago and I recall we would always pour the agar powder into the "broth" in an Erlenmyer an just keep it from clumping. Then when we autoclaved it the agar went into a colloidal soln.

THEN, the important part was to make the plates and store them upside down after setting down to 40 C(I think it was that high)
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fayorks
 
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Reply Fri 2 May, 2014 07:53 pm
Thanks farmerman,that was really helpful
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