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Sun 23 Aug, 2009 04:57 am
Artificial Life One Step Closer: Scientists Clone And Engineer Bacterial Genomes In Yeast And Transplant Genomes Back Into Bacterial Cells
Researchers at the J. Craig Venter Institute (JCVI), a not-for-profit genomic research organization, have just published results describing new methods in which the entire bacterial genome from Mycoplasma mycoides was cloned in a yeast cell by adding yeast centromeric plasmid sequence to the bacterial chromosome. Researchers modified it in yeast using yeast genetic systems. This modified bacterial chromosome was then isolated from yeast and transplanted into a related species of bacteria, Mycoplasma capricolum, to create a new type of M. mycoides cell.
This is the first time that genomes have been transferred between branches of life?from a prokaryote to eukaryote and back to a prokaryote. The research was published by Carole Lartigue et al in the journal Science on August 21.
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