8
   

how would i measure bacteria growth in a petri dish?

 
 
bacteriagirl97
 
  1  
Reply Sat 6 Feb, 2010 02:16 pm
@farmerman,
thank you! i will try to put it at a higher temp. but how would i do that?
tsarstepan
 
  1  
Reply Sat 6 Feb, 2010 02:16 pm
@dadpad,
dadpad wrote:

Quote:
also i have left the dishes out on my kitchen counter but nothing has grown yet and it has been a whole three days!


Let grow in undisturbed warm location, ideally in an environment around 100° F (37° C) - not in sunlight or on a heating register.

There you hit the nail on the head. She needed an incubator of sorts.
0 Replies
 
bacteriagirl97
 
  1  
Reply Sat 6 Feb, 2010 02:18 pm
@dadpad,
oh so sunlight is bad for it?
bacteriagirl97
 
  1  
Reply Sat 6 Feb, 2010 02:19 pm
@ossobuco,
it sat in the cold!
0 Replies
 
bacteriagirl97
 
  1  
Reply Sat 6 Feb, 2010 02:20 pm
@tsarstepan,
i already did and she doesn't know she suggested to go on here
0 Replies
 
bacteriagirl97
 
  1  
Reply Sat 6 Feb, 2010 02:20 pm
@Rockhead,
yup but i can't see much because there is musture inside that's blocking my veiw!
0 Replies
 
farmerman
 
  2  
Reply Sat 6 Feb, 2010 02:34 pm
@bacteriagirl97,
do you have a toaster oven?
tsarstepan
 
  1  
Reply Sat 6 Feb, 2010 02:36 pm
@farmerman,
Toaster oven? Perfect bit of Yankee ingenuity and thinking!
0 Replies
 
bacteriagirl97
 
  2  
Reply Sat 6 Feb, 2010 06:50 pm
I T'S GROWING!!! thank you all for your help! my dad helped me make an inkerbater by just putting the petri dishes in a shoe box and then just put a lamp on top of it! so because of the heat from the lamp the bacteria has grown!!!!! yay!
tsarstepan
 
  1  
Reply Sat 6 Feb, 2010 06:58 pm
@bacteriagirl97,
YAY! You GROW Girl! Bacteria that is....

Sorry that was a tad obnoxious.... Confused
0 Replies
 
dadpad
 
  2  
Reply Sat 6 Feb, 2010 07:06 pm
Arn't dads wonderful.


0 Replies
 
roger
 
  2  
Reply Sat 6 Feb, 2010 07:08 pm
@bacteriagirl97,
bacteriagirl97 wrote:

oh so sunlight is bad for it?


Yeah, that would fit my own limited understanding.
0 Replies
 
ossobuco
 
  1  
Reply Sat 6 Feb, 2010 08:52 pm
@rosborne979,
That's true, rosborne, re expectation of results. Good catch. (One of our best finds was from a result that made no sense to us..)
0 Replies
 
ossobuco
 
  1  
Reply Sat 6 Feb, 2010 08:55 pm
@bacteriagirl97,
Yaaaaaaaay!
Good dad thinking..
0 Replies
 
ScienceChick98
 
  1  
Reply Thu 17 Nov, 2011 12:49 am
@bacteriagirl97,
I recently did the same project, but i included the mouth of as cat, the reason nothing has grown is because it has to be in a warm and dark place, i found putting them in my closet the best spot, i started seeing colonies in about 2 days. Also make sure you have a piece of tape on each side to hold the lid on but still allowing a little air into it.
0 Replies
 
MaxJackson
 
  1  
Reply Sun 12 Feb, 2017 12:26 pm
@bacteriagirl97,
Try using an inoculating loop and the streak method. This way, the spit will be evenly distributed among the agar. Also, make sure to use an incubator at around 98.6 (normal human body temperature) for the human spit and 102.5 (normal dog body temperature) for the dog spit to simulate the normal temperatures. This way, you have an optimal chance of growth. Good luck!
0 Replies
 
josephdfox5
 
  0  
Reply Thu 4 Apr, 2019 01:26 pm
@bacteriagirl97,
The easiest way to measure bacterial growth is to put your sample on a clear glass plate under a microscope and count how many bacteria cells there are. Alternatively, you can measure turbidity, which is the amount of bacteria in your sample
0 Replies
 
 

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