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I need help designing a plasmid for luc-gene incorporation into yeasts

 
 
sbsnp
 
Reply Sun 9 Apr, 2017 04:55 am
I want to incorporate luciferase gene from fireflies into baker's yeast, and measure expression.

How do I find the exact gene name?
How do I find a suitable YI plasmid?
How do I find enzyme to

Is this gene the right one to use LOC106740757?
Can I use pYES2 modified vector with GAL1 promoter added?
How do I find specific enzymes
1. to cut the plasmid and insert the luc gene
2. to linearize the plasmid
3. to integrate the plasmid into the yeast genome
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