@Rawchester45,
True.
In a short summary, Restriction Fragment Length Polymorphisms are molecular markers that are utilized to identify two different-sized alleles of one locus (the pieces of broken DNA created by the enzyme, restriction endonuclease), which are then identified by probes (pieces of radio labelled DNA with nucleic acid that are similar to the marker fragments) utilized to detect it. The chromosomal mapping is done by making crosses between homozygous lines which reveal allelic differences for selected probes. Markers are put in order in order to see the relative genetic distances between them, and these markers are assigned to their linkage groups on the basis of the recombination values from all their pairwise combinations.