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Fri 14 Dec, 2012 03:16 am
I am very new at doing PAGE gels and everything else. I want to know in detail how to prepare and load 1 ug of protein sample per well to run a gel. I do have the concentration of the protein, and know that the wells probably hold about 14 ul safely. I am just confused about how to work out the dilutions to achieve 1 ug of the protein, with the loading dye and dilutant, PBS or TBS for example, or even DMEM if it is cell culture supernatant. Has anyone got a working example of these things including the calculation?
@disconbobulated,
Theres a "throwaway" free mag on Genetics and Genomiocs that is mostly ads but their section of "Omics" each week has methods and tips on running gels.
Its outta my field but the tips seem valuable. What dyes are you using?
stuff like ninhydrine?
@disconbobulated,
just the Nu Page commercial one, non reducing
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