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GTP and RNA

 
 
Reply Tue 25 Mar, 2008 01:33 am
ok suppose i want to take a short DNA strand like this one 5-gatcctgcta-3 into RNA, and want it to be labeled. So radioactive GTP is used. And supposed if the GTP is radioactived with the additon of 4th phosphate group in the alpha postion, will the resulting RNA be radiolabeled? What about the beta position?

I don't get this at all Sad
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Type: Discussion • Score: 1 • Views: 673 • Replies: 2
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Miller
 
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Reply Wed 26 Mar, 2008 04:07 pm
What course are you taking?
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Wolf ODonnell
 
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Reply Fri 28 Mar, 2008 04:08 pm
I don't understand. Why would you need to create your own radioactively labelled GTP? Surely they sell that stuff?
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