@Bob1607,
You are not going to like reading this. If you don't like me, feel free to click through to my profile and hit Ignore. I won't be offended. Here goes:
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When you start playing Junior CSI, it's all over but the shouting.
If my husband was trying to lab test my underwear, he'd be out on his ear, and for good reason, too (and we have been wed for over 25 years and he posts here and I love him till the end of time).
When you do not trust someone, your relationship is over. Full stop.
Right now, you're just happily playing detective. And to what end? Divorce is no-fault pretty much everywhere in the United States and in most western countries, or you can plead irreconcilable differences.
You don't need to prove cheating to get a divorce. You don't need it to get or share custody. You don't need it to get or minimize or eliminate spousal support, either.
Because what else is this bullshit leading to? You're going to confront her with the questionable stains on her panties and she'll decide to stay married to you? If you believe that, I have a bridge you might be interested in. It's cheap, but you'll have to move it yourself.
If she is cheating, it is probably over. If she isn't and you are falsely accusing her (and you are certainly going to drastic measures with little more than a few bits of body language and some hunches), then it's over. If you don't trust her (you don't), then it's over.
And consider the fine and dandy conversation you are going to have with your children when they find out what is going on - because they will. If their mother is cheating, they won't be so happy with her. But your pretend Sherlock act isn't going to endear you to them, either.
Consider your options and have a conversation with your wife. You know, like civilized people do. If your marriage is done, then work out how best you can co-parent your children, who are innocent in all of this. And please don't "stay together for the children". That doesn't do your offspring any favors. All it does is teach them that marriage sucks and love doesn't matter as much as saving money does. Some lesson, eh?
Oh, and a few carriage returns would be nice.
I will now go and yell at some kids to get off my lawn.