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How can we get rid of bacterial clump formation in Agrobacterium (GV2260) culture?

Forums: Microbiology
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Saber DE
 
Reply Fri 5 Jun, 2015 06:04 am
I have tried YEB, YEM, LB mediums but still facing the problem. temrature: 28c; rpm: tried 190 and 230; antibiotic rifampisin(tried 10, 25 and 50mg/l) and kanamycin(50mg/l)

also I tried culture without antibiotic still I had the problem. 

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