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Member since June 23, 2006
theologian
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PCR and DNA LIGASE
Fri 23 Jun, 2006 07:51 pm -
Hi I am doing a Site Directed Mutagenesis and am wondernig why DNA LIGASE is not necsry after I do a PCR to amplify the dsDNA strands. Are the mutated prmiers not nicked one PCR is done, dont they...
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