MDR1-MDCK Permeability Assay

An investigational drug should be evaluated in vitro to determine whether they are a potential substrate of P-glycoprotein (a well-recognized efflux transporter in many tissues and organs such as brain, kidney and intestine). MDR1-MDCK permeability assay is one of drug absorption screening methods. This method uses MDR1-MDCK cell line which is a combination of Madin Darby Canine Kidney (MDCK) cells and MDR1 gene to encode the efflux protein, P-gp. The MDR1-MDCK permeability assay is a valuable tool to identify and characterize P-gp substrates and inhibitors, it can help understand the mechanism of drug efflux and highlight early potential issues with drug permeability at an early stage.