CFSE proliferation peaks

Reply Fri 4 Aug, 2017 12:42 am
I am observing cell proliferation kinetics of B cells using CFSE staining. If I stain quiescent cells with CFSE and chase them for 3-6 days, they show distinct peaks for different generations. However, if I pulse already activated (but previously unstained) cells on day 3 or 6, I get a broad-spread peak, instead of distinct peaks. Is the multiple peak profile specific only for quiescent or synchronous cells?
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