the definite article

Ifi you need to clarify

A. "Can the two of you .." is the better choice.
Although eliminating 'the' doesn't' make it wrong grammatically, it makes it awkward. As a spoken sentence, it wouldn't be something you'd be likely to hear.

Both a) and b) are possible. Use of articles is very context-dependent. If there are only two people there, the "two" is definite, so use "the." If there is a larger group of people and you just want two of them to help, use b).

a) Can the two of you... (speaking to the only two people there)
b) Can two of you... (speaking to a larger group)

Dear friends and teachers,

I got it. Thanks for your attention!

Excellent!

Of the course.